This script will predict the secondary structure from miRNA hairpin and combine all structure in a single file with their name.

install prerequisite

1.ghostscript

sudo apt-get install ghostscript

2.pdftk

sudo apt-get install pdftk

3.RNAfold

https://www.tbi.univie.ac.at/RNA/

Running the script

1. Change the name of file containing hairpin sequences for miRNA in mirna_structure_prediction.sh in line 5 and 16 and run the script as

bash mirna_structure_prediction.sh

2. Copy the content of file 'precurssor_name' in combing_pdf.sh

3. run the main script as

bash combing_pdf.sh